Anti-WBP4 Rabbit Polyclonal Antibody for WB, ELISA - O75554

Core Product Specifications and Parameters

Biological Background: WBP4 Function and Localization

• WBP4 (WW domain-binding protein 4), also known as Formin-binding protein 21 (FBP21), is a component of the spliceosome involved in pre-mRNA splicing.
  Related references:
  PMID:19592703 PMID:28781166 PMID:9724750
• It may play a role in cross-intron bridging of U1 and U2 snRNPs in the mammalian A complex, contributing to early spliceosome assembly.
  Related references:
  PMID:9724750
• The protein localizes to the nucleus and is enriched in nuclear speckles, sites of active pre-mRNA processing.
• As indicated by its keywords, WBP4 has zinc-finger domains, undergoes phosphorylation, and is associated with intellectual disability when mutated.
• Alternative splicing of WBP4 produces multiple isoforms, which may have distinct roles in splicing regulation.
• Its molecular weight is approximately 42.5 kDa (UniProt), with a reported detection band at 43 kDa on Western blots.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the full-length human WBP4 protein (amino acids 1–376), which covers the entire sequence; the antibody is expected to recognize multiple isoforms that share this region.
• For Western blot, a specific band at approximately 43 kDa is detected; consider validating antibody specificity using WBP4-overexpressing lysates or siRNA knockdown controls.
• In ELISA, the antibody can be used for direct or indirect detection of WBP4; ensure proper coating concentration and antibody dilution optimization for the target sample matrix.
• Given the reported rat cross-reactivity, this antibody may be suitable for studies in rodent models, but validation in your specific experimental system is recommended.
• Due to WBP4’s role in splicing, consider analyzing its expression in proliferating or actively transcribing cell types and tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver comprehensive IVD raw material supply solutions. This WBP4 polyclonal antibody serves as a reliable reagent for splicing-related research and assay development. Our platform offers bulk antibody sourcing, validated antibody pairs, and expert technical support to accelerate your diagnostic assay development and biomarker discovery programs.