Anti-WBP2NL Rabbit Polyclonal Antibody for WB - Q6ICG8

Core Product Specifications and Parameters

Biological Background: WBP2NL Function and Localization

• WBP2NL (WW domain-binding protein 2-like), also known as PAWP (postacrosomal sheath WW domain-binding protein), is a protein encoded by the WBP2NL gene in humans.
• It is predominantly expressed in testis, suggesting a specialized role in male reproductive biology.
• Functional studies indicate that WBP2NL may play a role in meiotic resumption and pronuclear formation during fertilization, mediated by a WW domain-signaling pathway.
• The protein is characterized by the presence of WW domain-binding motifs and contains repetitive sequence elements, as reflected by its keyword annotation "Repeat".
• According to its UniProt record, WBP2NL is a reference proteome member and has been identified in proteomics studies.
• The calculated molecular weight of the unmodified protein is approximately 31.9 kDa, though the native form may run at ~32 kDa due to potential modifications.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated using a recombinant immunogen corresponding to the N-terminal region (aa 1-120) of human WBP2NL. It is expected to recognize full-length WBP2NL in denaturing and native conditions.
• For Western blotting, a band around 32 kDa should be observed; optimal dilution and blocking conditions should be empirically determined for each sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), appropriate fixation and permeabilization protocols should be used to preserve the epitope, and co-staining with subcellular markers may help reveal localization patterns.
• Although the antibody cross-reacts with mouse and rat orthologs according to the supplier, we recommend validating reactivity and specificity in each species and target tissue independently.
• ELISA applications are possible; consider immobilizing the recombinant immunogen as a positive control and titrating the antibody to establish a suitable working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-WBP2NL rabbit polyclonal antibody supports investigations into fertilization mechanisms, meiotic regulation, and reproductive biology. In addition to high-quality primary and secondary antibodies, CamelBio can assist with sourcing validated antibody pairs, monoclonal alternatives, bulk ancillary reagents, and rare target raw materials to accelerate your assay development and research projects.