Anti-WAVE2/WASF2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y6W5

Core Product Specifications and Parameters

Biological Background: WAVE2/WASF2 Function and Localization

• WAVE2/WASF2, also known as Protein WAVE-2, Verprolin homology domain-containing protein 2, and Wiskott-Aldrich syndrome protein family member 2, is encoded by the WASF2 gene.
• As a member of the WASP family, WAVE2 is an actin-binding protein that promotes formation of actin filaments downstream of tyrosine kinase and small GTPase signaling.
• It is a core component of the WAVE regulatory complex (WRC), which activates the Arp2/3 complex to drive branched actin network assembly essential for lamellipodia formation.
• Subcellularly, WAVE2 localizes to the cytoplasm, cytoskeleton, and specifically concentrates at lamellipodia and basolateral cell membranes.
• Post-translational modifications include phosphorylation, consistent with its role in signal transduction, and the protein has been identified in proteomic studies.
• WAVE2 is broadly expressed across human tissues, with highest levels in placenta, lung, and peripheral blood leukocytes, but is absent from skeletal muscle.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.
• Keywords also indicate involvement in host-virus interactions, highlighting potential relevance in infection biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the C-terminal portion of WAVE2, which may be conserved across species; researchers should validate reactivity in mouse and rat samples.
• For Western blot, the predicted molecular weight is approximately 54 kDa; use appropriate gel percentage and transfer conditions, and include positive and negative controls from relevant cell lines or tissues.
• In IHC-P, antigen retrieval and blocking steps should be optimized; test dilutions on known positive tissues like placenta or lung, which express high levels of WAVE2.
• For ELISA, pairing with a capture antibody may require screening for compatible pairs to build sensitive assays; consider the target's intracellular localization when preparing samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, from concept to clinic. Our WAVE2/WASF2 antibody supports investigations into actin dynamics, lamellipodia biology, and related signaling pathways. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare‑target raw materials to accelerate your assay development.