Anti-WASP Rabbit Polyclonal Antibody for WB, ELISA - P42768

Core Product Specifications and Parameters

Biological Background: WASP (WASp) Function and Localization

• WASP is an effector protein for Rho-type GTPases that regulates actin filament reorganization via interaction with the Arp2/3 complex. Related references: PMID:12235133 PMID:12769847 PMID:16275905
• It is important for efficient actin polymerization. Related references: PMID:12235133 PMID:16275905 PMID:8625410
• It serves as a possible regulator of lymphocyte and platelet function. Related references: PMID:9405671
• Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. Related references: PMID:18650809
• Promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Related references: PMID:20574068
• Promotes homologous recombination repair by promoting nuclear actin polymerization and driving motility of double-strand breaks. Related references: PMID:29925947
• Localizes to the cytoplasm, cytoskeleton, and nucleus.
• Expressed predominantly in the thymus, with lower expression in the spleen.
• Associated with Wiskott-Aldrich syndrome, contains phosphoprotein and repeat domains.

Experimental Guidance and Technical Tips

• For Western blot analysis, expect a band at approximately 53 kDa; consider using nuclear and cytoplasmic extracts to verify subcellular localization.
• In ELISA, the antibody may be utilized as a capture or detection reagent; validate performance in the relevant sample matrix, such as human cell lysates or serum.
• As a polyclonal antibody, assess potential cross-reactivity with related proteins; always confirm specificity in human-derived samples using negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with validated IVD raw materials, including polyclonal and monoclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents. For projects targeting the actin nucleation-promoting factor WAS, we offer one-stop sourcing of validated antibody pairs, optimized antibodies for immunoassays, and bulk reagent supplies. Our team also assists with rare-target raw-material procurement to accelerate your assay development from concept to clinic.