Anti-WAPL Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q7Z5K2

Core Product Specifications and Parameters

Biological Background: WAPL Function and Localization

• WAPL (Wings apart-like protein homolog), also known as friend of EBNA2 and WAPL cohesin release factor, is a critical regulator of sister chromatid cohesion and chromosome segregation during mitosis.
• It negatively regulates cohesin association with chromatin, ensuring proper removal of cohesin from chromosome arms in prophase and promoting accurate sister chromatid resolution. Related references: PMID:26299517
• The protein couples DNA replication to sister chromatid cohesion, which is essential for faithful chromosome partitioning in both mitotic and meiotic cells, and also contributes to DNA repair processes.
• WAPL localizes predominantly to the nucleus and chromosomes, but is also detected in the cytoplasm.
• Isoform 1 is highly expressed in uterine cervix tumors, while isoform 2 is widely expressed with particularly high levels in skeletal muscle and heart.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100), which is conserved in human WAPL isoforms; consider using peptide competition assays to validate antibody specificity.
• For Western blot, the predicted molecular weight is approximately 133 kDa; use appropriate gel percentages and transfer conditions to ensure efficient detection of this relatively large protein.
• For immunofluorescence and immunoprecipitation, optimize fixation and lysis conditions in your experimental system, and include appropriate controls (e.g., isotype control, knockdown/knockout validation).
• Cross-reactivity with mouse is reported; verify performance in the relevant species and tissue context before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers and research teams with streamlined access to critical raw materials, including this WAPL rabbit monoclonal antibody for cohesin biology and cell cycle research. Our portfolio covers validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, all supported by technical consultation to de-risk your assay development. From early‐stage concept to clinical application, we bridge the gap between target discovery and reliable diagnostic manufacturing.