Anti-VWC2L Polyclonal Antibody for WB and ELISA - Q505H4

Core Product Specifications and Parameters

Biological Background: VWC2L Function and Localization

• VWC2L (von Willebrand factor domain-containing protein 2-like), also known as Brorin-like, is a secreted protein containing a von Willebrand factor type C (VWC) domain.
• It is implicated in neurogenesis and bone metabolism, with evidence suggesting a role in promoting matrix mineralization (PubMed:22209847). Related references: PMID:22209847
• In preosteoblastic cells, VWC2L weakly but significantly inhibits BMP2 and BMP6 activity, indicating a modulatory function in bone morphogenetic protein signaling (PubMed:19852960). Related references: PMID:19852960
• The protein is predominantly expressed in the brain, with additional detection in bone (femur, calvaria), heart, lung, and kidney. Isoform-specific expression patterns vary across tissues.
• Subcellularly, VWC2L is found in the extracellular space (secreted) and at synapses, consistent with roles in intercellular signaling.
• Multiple isoforms are generated through alternative splicing, contributing to tissue-specific functions.
• Keywords highlight its involvement in development and its structural repeat motif.

Experimental Guidance and Technical Tips

• The antibody is raised against an N-terminal synthetic peptide (aa 1-50) of mouse VWC2L. For WB, target detection at approximately 25 kDa, but consider potential isoform variations due to alternative splicing.
• For ELISA, the peptide immunogen may be used as a positive control; titrate antibody concentration for optimal sensitivity.
• Cross-reactivity is reported for human and mouse; validate in the relevant sample system if using other species.
• For bone mineralization or neurogenesis studies, consider using brain or bone tissue lysates; note the predominant brain expression and lower expression in other tissues.
• Given the secreted and synaptic localization, consider using appropriate subcellular fractionation protocols if analyzing protein distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner for diagnostic manufacturers, labs, and research institutes. This anti-VWC2L polyclonal antibody supports studies in neurobiology and bone biology. Beyond single reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and offer rare target raw-material sourcing to streamline your development pipeline.