Anti-VTI1A Rabbit Polyclonal Antibody for WB, ELISA - Q96AJ9

Core Product Specifications and Parameters

Biological Background: VTI1A Function and Localization

• VTI1A (Vesicle transport through interaction with t-SNAREs homolog 1A) is a v-SNARE protein also known as Vti1-rp2 or Vti1-like 2, encoded by the VTI1A gene in humans.
• It mediates vesicle transport pathways through specific interactions with t-SNAREs on target membranes, contributing to vesicle trafficking specificity and lipid bilayer fusion.
• Functionally, VTI1A is involved in retrograde transport from late endosomes to the trans-Golgi network.
• In cooperation with VAMP7, it participates in an unconventional RAB1-dependent trafficking route to the cell surface, utilized by potassium channel interacting proteins KCNIP1 and KCND2.
• VTI1A may also play a role in increased cytokine secretion associated with cellular senescence.
• Subcellularly, it localizes to cytoplasmic vesicles and the Golgi apparatus membrane.
• The protein is predicted to contain a transmembrane helix and a coiled-coil domain, characteristic of SNARE proteins.
• Keywords also highlight that VTI1A undergoes alternative splicing and is involved in protein transport.

Experimental Guidance and Technical Tips

• The immunogen was designed using the N-terminal region (amino acids 1–180), which may allow recognition of both full-length and potential isoform variants if they share this region; however, always validate specificity in your experimental system.
• Recommended for Western blotting (WB) and ELISA. For WB, the predicted molecular weight is ~25 kDa, but confirm migration with your samples.
• The antibody shows cross-reactivity with human, mouse, and rat, making it suitable for detecting VTI1A orthologs in these species; validate in the relevant sample type.
• Given VTI1A’s localization to Golgi and vesicles, subcellular fractionation or immunofluorescence might be feasible, though not validated in the current product specification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This VTI1A polyclonal antibody is part of our expanding portfolio for vesicle trafficking and SNARE protein research, offering validated tools for Western blotting and ELISA. In addition to high-quality antibodies, we supply optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and handle rare target raw-material sourcing to accelerate your assay development.