Anti-VSNL1/VILIP-1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P62760

Core Product Specifications and Parameters

Biological Background: VSNL1/VILIP-1 Function and Localization

• VSNL1, also known as Visinin-like protein 1 or HLP3, belongs to the visinin/recoverin family of neuronal calcium sensor proteins.
• It functions as a calcium-dependent regulator, with in vitro studies showing its role in inhibiting rhodopsin phosphorylation.
• VSNL1 is highly expressed in the brain and retina, exhibiting neuron-specific localization throughout the central and peripheral nervous systems.
• At the protein level, VSNL1 levels are significantly elevated in the cerebrospinal fluid of Alzheimer's disease patients, highlighting its potential as a biomarker.
• The protein contains EF-hand metal-binding domains and undergoes myristoylation, which mediates calcium-dependent membrane association.
• UniProt keywords for VSNL1 include calcium, metal-binding, lipoprotein, myristate, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 2–191 of human VSNL1, covering the N-terminal region that is highly conserved, contributing to cross-reactivity with mouse and rat.
• For Western blotting, a specific band at approximately 22 kDa is anticipated; consider optimizing blocking and antibody dilution in neuronal tissue lysates.
• In immunofluorescence/immunocytochemistry, appropriate fixation and permeabilization conditions should be empirically determined for neuronal cell types.
• For ELISA applications, indirect or sandwich formats may be explored, with coating conditions optimized for the recombinant immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this high-quality rabbit monoclonal antibody against VSNL1/VILIP-1, ideal for neuroscience and neurodegenerative disease research. We provide validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions to support your diagnostic development from concept to clinic. Contact CamelBio to expedite your procurement with reliable, one-stop supply services.