Anti-VSNL1/VILIP-1 Rabbit Monoclonal Antibody for WB, ELISA - P62760

Core Product Specifications and Parameters

Biological Background: VSNL1/VILIP-1 Function and Localization

• VSNL1/VILIP-1 (Visinin-like protein 1), also known as Hippocalcin-like protein 3 (HLP3), is a neuron-specific calcium-binding protein predominantly expressed in the brain and retina.
• It belongs to the visinin/recoverin subfamily of EF-hand calcium sensor proteins, possessing Ca²⁺-binding EF-hand repeats that mediate conformational changes upon calcium binding.
• In vitro, it regulates the inhibition of rhodopsin phosphorylation in a calcium-dependent manner, suggesting a role in phototransduction or calcium-mediated signal modulation.
• Protein levels are increased in the cerebrospinal fluid of Alzheimer disease patients, highlighting its potential as a biomarker for neurodegeneration.
• The protein contains N-terminal myristoylation and lipoprotein motifs, indicating membrane targeting capabilities that may be regulated by calcium binding.
• With a molecular weight of approximately 22 kDa, VSNL1/VILIP-1 is a compact calcium sensor amenable to detection by standard Western blotting techniques.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length protein (amino acids 2-191), covering conserved EF-hand domains; for Western blot, consider using brain or retinal tissue lysates where endogenous expression is high.
• Cross-reactivity with mouse and rat extends applicability to rodent models of neurological disease; validate detection in relevant sample systems.
• For ELISA, the recombinant immunogen can serve as a positive control; optimize coating and detection conditions for best performance.
• The predicted molecular weight is ~22 kDa; use a 12–15% SDS-PAGE gel for optimal separation and transfer in Western blotting.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency, but each new application should be independently optimized.

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CamelBio provides a one-stop sourcing platform for IVD raw materials, empowering diagnostic developers with high-quality antibodies like this anti-VSNL1/VILIP-1 monoclonal antibody for neuroscience and neurodegeneration research. Our comprehensive supply chain covers validated antibody pairs, bulk ancillary reagents, and procurement for rare targets, ensuring a seamless transition from R&D to clinical application.