Anti-VSIG1 Rabbit Polyclonal Antibody for WB, ELISA - Q86XK7

Core Product Specifications and Parameters

Biological Background: VSIG1 Function and Localization

• VSIG1 (V-set and immunoglobulin domain-containing protein 1) is a transmembrane protein belonging to the immunoglobulin superfamily, characterized by extracellular V-set and immunoglobulin-like domains.
• It is identified as the cell surface glycoprotein A33 antigen (GPA34), implicated in cell adhesion or signaling based on its domain architecture.
• The protein is anchored at the plasma membrane via a single-pass transmembrane helix, with the Ig domains exposed extracellularly.
• Expression is highly tissue-specific: detected primarily in stomach mucosa and testis, with minor expression in pancreas at the protein level; notably, it is found in a significant fraction of gastric, esophageal, and ovarian cancers.
• Multiple isoforms are produced by alternative splicing, as indicated by UniProt annotation.
• The extracellular region contains predicted disulfide bonds and N-glycosylation sites, typical of Ig-domain proteins, which may contribute to structural stability and function.
• Keywords: Alternative splicing, Disulfide bond, Glycoprotein, Immunoglobulin domain, Membrane, Proteomics identification, Reference proteome, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 50-230) encompassing the Ig domains; thus, the antibody is expected to recognize the native extracellular epitope, suitable for ELISA and non-denaturing applications.
• For Western blot, use reducing conditions to detect the ~42 kDa band; include positive controls such as stomach or testis lysates from human, mouse, or rat.
• Validate species cross-reactivity by testing lysates from mouse and rat tissues known to express VSIG1.
• In ELISA, the recombinant protein immunogen can serve as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with validated antibodies and ancillary reagents. This VSIG1 rabbit polyclonal antibody, targeting a cancer- and tissue-restricted antigen, supports gastrointestinal and oncology research. Beyond single reagents, we facilitate access to custom monoclonal/polyclonal antibody development, bulk buffer components, and rare target raw-material procurement, streamlining your path from concept to clinic.