Anti-VPS54 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P1Q0

Core Product Specifications and Parameters

Biological Background: VPS54 Function and Localization

• VPS54 (Vacuolar protein sorting-associated protein 54) is also known as hepatocellular carcinoma protein 8 (HCC8), tumor antigen SLP-8p, and PPP1R164; it is a component of the GARP (Golgi-associated retrograde protein) complex.
• Acts as a component of the GARP complex that mediates retrograde transport from early and late endosomes to the trans-Golgi network (TGN).
• The GARP complex is required for the cycling of mannose 6-phosphate receptors between the TGN and endosomes, which is essential for proper lysosomal sorting of acid hydrolases such as CTSD.
• Within the GARP complex, VPS54 is required to tether the complex to the TGN; it is not involved in endocytic recycling. Related references: PMID:18367545 PMID:25799061
• Subcellular localization: Golgi apparatus, trans-Golgi network membrane.
• Post-translational modifications and features: phosphoprotein, contains coiled coil domains, and is subject to alternative splicing.
• Predicted molecular weight is approximately 111 kDa (UniProt annotation: 110,589 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420–720, a region likely encompassing coiled-coil motifs important for complex assembly; consider using this epitope information when designing competitive assays or truncation studies.
• For Western blotting, a dilution series may be required to detect endogenous VPS54 at the predicted molecular weight of 111 kDa; validate in the relevant sample system.
• In immunofluorescence/ICC, fixation and permeabilization conditions should be optimized to preserve Golgi/TGN membrane localization; co-staining with TGN markers (e.g., TGN46) is recommended.
• ELISA can be applied for quantitative detection; ensure recombinant protein or peptide controls match the immunogen region for accurate quantification.
• Cross-reactivity with human and mouse has been confirmed; for other species, perform sequence alignment and preliminary testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to premium IVD raw materials. This anti-VPS54 polyclonal antibody supports investigations into Golgi/endosome trafficking and tumor antigen biology. Through our platform, you can efficiently source validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and even rare-target raw materials, streamlining your development from concept to clinic.