Anti-VPS4A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UN37

Core Product Specifications and Parameters

Biological Background: VPS4A Function and Localization

• Vacuolar protein sorting-associated protein 4A (VPS4A) is an AAA ATPase that utilizes ATP hydrolysis to disassemble ESCRT-III complexes, essential for membrane fission events in the endosomal pathway.
• It is required for multivesicular body (MVB) formation and intraluminal vesicle (ILV) sorting; VPS4A redistributes ESCRT-III components to the cytoplasm for reuse, regulating endosome size, primary cilium organization, and nuclear envelope sealing during anaphase (PubMed:33186545). Related references: PMID:33186545
• Plays a central role in the abscission step of cytokinesis; retained at the midbody by ZFYVE19/ANCHR and CHMP4C until checkpoint termination, VPS4A release then triggers membrane scission (PubMed:24814515). Related references: PMID:24814515
• Mediates the exosomal release of SDCBP, CD63, and syndecan, linking the ESCRT machinery to exosome biogenesis (PubMed:22660413). Related references: PMID:22660413
• Critical for normal erythroblast cytokinesis and erythropoiesis; mutations are associated with congenital dyserythropoietic anemia (PubMed:33186543). Related references: PMID:33186543
• In conjunction with the ESCRT machinery, VPS4A participates in topologically similar membrane fission events that are hijacked by enveloped viruses (e.g., HIV-1) for budding.
• Subcellularly localizes to late endosome membranes, the midbody, cytoplasm, and mitotic spindle.
• Ubiquitously expressed, with links to disease variants including cataracts, hereditary hemolytic anemia, and intellectual disability (as listed in UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1-120 of human VPS4A, an N-terminal region; this may facilitate cross-reactivity with mouse and rat orthologs.
• As a polyclonal antibody, it generally provides robust detection in denaturing (WB) and native (IHC-P, IF/ICC) applications; however, optimal working dilutions should be determined empirically for each assay.
• For Western blot, an expected band of approximately 49 kDa is observed; use whole-cell or tissue lysates expressing VPS4A.
• For IHC-P and IF/ICC, validate specificity with appropriate positive and negative controls, and optimize antigen retrieval or permeabilization conditions.
• In ELISA, consider pairing this antibody with other VPS4A antibodies to build a sandwich assay after validation.
• Since VPS4A localizes to the midbody during cytokinesis, synchronizing cells at late mitosis or enriching for midbody structures may enhance detection for IF/ICC.
• Because VPS4A is ubiquitously expressed, verify knockdown or knockout efficiency in RNAi/CRISPR experiments to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a comprehensive one-stop supply of IVD raw materials, technical support, and consulting services from concept to clinic. This VPS4A polyclonal antibody supports research into endosomal sorting, cell division, and viral pathogenesis. For bulk reagent sourcing, validated antibody pairs, or customized antibody development, CamelBio offers streamlined access to rare and routine targets.