Anti-VPS39 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96JC1

Core Product Specifications and Parameters

Biological Background: VPS39 Function and Localization

• VPS39, also known as hVam6p, TLP, or VAM6, is a Vam6/Vps39-like protein that functions as a regulator of TGF-beta/activin signaling. It inhibits SMAD3-dependent transcription while activating SMAD2-dependent transcription by interfering with SMAD3/SMAD4 complex formation.
• As a core component of the HOPS endosomal tethering complex, VPS39 plays a critical role in vesicle-mediated protein trafficking to lysosomal compartments, including endocytic transport and autophagy. The HOPS complex is recruited to Rab7 on late endosomal membranes and is essential for fusion of endosomes and autophagosomes with lysosomes. Related references: PMID:23351085, PMID:11448994, PMID:23167963, PMID:25783203, PMID:37821429
• VPS39 localizes primarily to the cytoplasm, lysosome membrane, and late endosome membrane.
• The protein is widely expressed, with highest levels observed in heart, skeletal muscle, kidney, pancreas, brain, placenta, and spleen.
• Keywords from UniProt include alternative splicing, autophagy, endosome, lysosome, protein transport, and host-virus interaction, reflecting its multifaceted roles in cellular trafficking and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 606-875) of human VPS39, which is highly conserved across human, mouse, and rat, likely contributing to the observed cross-reactivity.
• This antibody has been applied in WB, IF/ICC, and ELISA; consider optimizing dilutions and controls for your specific experimental setup.
• Given the target's involvement in endosomal trafficking and signaling, the antibody may be useful in studying endosome maturation, autophagy flux, and TGF-beta pathway modulation.

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