Anti-VPS37B Polyclonal Antibody for WB, ELISA - Q9H9H4

Core Product Specifications and Parameters

Biological Background: VPS37B Function and Localization

• VPS37B is a component of the ESCRT-I complex, regulating vesicular trafficking and endosomal sorting of ubiquitinated cargo into multivesicular bodies.
• It is also known as vacuolar protein sorting-associated protein 37B (ESCRT-I complex subunit VPS37B).
• The protein is localized to late endosome membranes, consistent with its role in endocytic sorting.
• VPS37B is widely expressed across tissues, with notable expression in macrophages and lymphocytes.
• Functional studies suggest a potential role in cell growth and differentiation.
• Key post-translational modifications include methylation, and it has been identified through proteomics (UniProt keywords: Methylation, Proteomics identification).
• The molecular weight is approximately 31 kDa (UniProt: 31307 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the N-terminal portion of VPS37B (amino acids 60-170). Consider validating antibody specificity using positive and negative control lysates.
• For Western blot, the predicted band size is ~31 kDa; run appropriate molecular weight markers and optimize loading.
• This polyclonal antibody may detect endogenous VPS37B across human, mouse, and rat samples; consider species-specific validation.
• Use standard ELISA protocols, optimizing antibody dilution and coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For studies involving the ESCRT-I complex and endosomal trafficking, our VPS37B rabbit polyclonal antibody offers a reliable research tool, supported by our comprehensive sourcing services that cover everything from bulk supply to custom rare-target raw materials.