Anti-VPS37A Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NEZ2

Core Product Specifications and Parameters

Biological Background: VPS37A Function and Localization

• VPS37A, also known as HCRP1 (hepatocellular carcinoma-related protein 1), is a component of the ESCRT-I complex, a key regulator of the vesicular trafficking pathway.
• It functions in the sorting of ubiquitinated endocytic cargo into multivesicular bodies (MVBs), facilitating the degradation of transmembrane receptors and other proteins.
• The protein may also play a role in cell growth and differentiation, consistent with its decreased expression in hepatomas (hepatocellular carcinomas).
• Subcellularly, VPS37A is localized to the late endosome membrane and can also be found in the nucleus.
• It is widely expressed across human tissues including heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, with notably higher abundance in the liver.
• VPS37A is subject to alternative splicing and is classified as a phosphoprotein, indicating regulatory post-translational modifications.
• Mutations in the VPS37A gene have been associated with hereditary spastic paraplegia (SPG53), a neurodegenerative disorder, and the protein is implicated in liver cancer, highlighting its biomedical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 224-397) of human VPS37A; this design may contribute to the antibody’s cross-reactivity with mouse and rat homologs.
• For Western blotting, a band at approximately 44 kDa is expected; however, due to alternative splicing and phosphorylation, multiple bands or shifts may be observed in some samples.
• In IF/ICC experiments, staining consistent with late endosome and nuclear localization should be anticipated; co-staining with organelle markers is recommended for validation.
• ELISA can be employed for antibody titer quantification or antigen detection; optimal coating and detection conditions should be determined empirically.
• Given the decreased expression in hepatomas, comparative analysis between normal and tumor liver tissue samples may be of interest when using this antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and labs. For researchers studying endosomal sorting and liver cancer biology, we provide paired VPS37A detection reagents, optimized polyclonal antibodies, and ancillary raw materials in bulk. Our sourcing solutions support your assay development from concept to clinic.