Anti-VPS35 Rabbit Polyclonal Antibody for WB, ELISA - Q96QK1

Core Product Specifications and Parameters

Biological Background: VPS35 Function and Localization

• VPS35 (Vacuolar protein sorting-associated protein 35) is a core component of the retromer cargo-selective complex (CSC), essential for preventing lysosomal degradation of selected transmembrane cargo proteins; it is required for retrograde transport of IGF2R and SLC11A2 from endosomes to the TGN and for pIgR-pIgA transcytosis. Related references: PMID:15078903, PMID:15247922, PMID:20164305
• The retromer CSC interacts with distinct sorting nexins (SNX3, SNX27, SNX-BAR) to define specialized trafficking pathways; for example, SNX3-retromer mediates retrograde endosome-to-TGN transport of WLS. Related references: PMID:30213940
• VPS35 facilitates the recruitment of the WASH complex and TBC1D5 to endosomal membranes, linking retromer function to actin polymerization and regulatory protein cycling. Related references: PMID:22070227, PMID:28892079, PMID:20923837
• Subcellular localization includes the cytoplasm, membrane, and endosomal compartments (early and late endosomes), consistent with its role in intracellular trafficking.
• Post-translational modifications include phosphorylation (keyword "Phosphoprotein"), suggesting regulatory control of retromer assembly and cargo sorting.
• Tissue expression is ubiquitous, with high levels in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney, and colon.
• Mutations in VPS35 are associated with autosomal-dominant late-onset Parkinson disease (PARK17), implicating retromer dysfunction in neurodegeneration.
• Additionally, VPS35 plays a role in host-virus interactions; the retromer CSC mediates the exit of human papillomavirus from early endosomes during infection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-250) of human VPS35, which is highly conserved; cross-reactivity with mouse is confirmed, and reactivity with other species may be possible given sequence homology.
• For Western blot (WB), a band of approximately 92 kDa is expected. Given the protein’s ubiquitous expression, positive signals can be anticipated in various human and mouse cell lysates (e.g., HeLa, HEK293, or mouse brain tissue); validate in your specific sample system.
• In ELISA, consider using either direct coating of recombinant VPS35 or capture assays; titrate the antibody to establish optimal working dilutions.
• For immunofluorescence or immunohistochemistry, note that this is a polyclonal antibody; additional validation may be required due to potential cross-reactivity with other VPS family members or retromer components.
• Store at -20°C in aliquots and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, supplying researchers and diagnostic developers with validated antibody reagents for intracellular trafficking and neurodegeneration targets like VPS35. Beyond this anti-VPS35 polyclonal antibody, we offer curated portfolios of monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents for assay development. For rare or customized targets, our sourcing network can help secure specialized raw materials to accelerate your project from concept to clinic.