Anti-VPS29 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBQ0

Core Product Specifications and Parameters

Biological Background: VPS29 Function and Localization

• VPS29 functions as a core component of the retromer cargo-selective complex (CSC) and the retriever complex, essential for endosomal recycling of transmembrane cargos and preventing their lysosomal degradation. Related references: PMID:37172566, PMID:39587083, PMID:38062209, PMID:28892079
• Within the retromer complex, it participates in retrograde transport from endosomes to the trans-Golgi network (TGN) and endosome-to-plasma membrane recycling, interacting with SNX-BAR, SNX3, and SNX27 subcomplexes. Related references: PMID:15247922, PMID:21725319, PMID:23563491
• As part of the retriever complex, VPS29 drives the retrieval and recycling of NxxY-motif-containing cargo proteins via coupling to SNX17, crucial for cell migration, adhesion, and signaling. Related references: PMID:28892079, PMID:39587083
• It is also involved in GLUT1 endosome-to-plasma membrane trafficking, dependent on association with ANKRD27. Related references: PMID:24856514
• Additionally, VPS29 mediates the exit of human papillomavirus from early endosomes during infection.
• Subcellularly, VPS29 localizes to the cytoplasm and is associated with endosome membranes, including early and late endosomes.
• Tissue expression is ubiquitous, with high levels in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon, skeletal muscle, kidney, and brain.
• Key post-translational modifications include acetylation, and the protein binds zinc, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen design targets the full-length (aa 1-186) human VPS29, potentially enhancing recognition of native conformations; consider this when optimizing assay conditions.
• For WB and IF/ICC, validate the antibody in your specific sample system, noting the ubiquitous but variable tissue expression of VPS29.
• In IF/ICC experiments, expect endosomal membrane staining; co-staining with early/late endosome markers may aid localization studies.
• ELISA performance should be confirmed under your laboratory’s standard protocols, as supplier validation data may not be available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For VPS29-related endosomal recycling and retromer studies, we offer validated antibody pairs, optimized polyclonal antibodies like this VPS29 Rabbit pAb, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to bridge your research from concept to clinic with our comprehensive supply capabilities.