Anti-VPS28 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UK41

Core Product Specifications and Parameters

Biological Background: VPS28 Function and Localization

• VPS28 is the human homolog of yeast Vps28, also designated as ESCRT-I complex subunit VPS28.
• It functions as a core component of the ESCRT-I machinery, which orchestrates vesicular trafficking, including multivesicular body (MVB) formation and cargo sorting.
• Within the ESCRT-I complex, VPS28 plays a role in recognizing and sorting ubiquitinated membrane proteins into intraluminal vesicles of late endosomes.
• Subcellularly, VPS28 resides at the cell membrane and on the late endosome membrane, aligning with its trafficking functions.
• Post-translational modifications such as acetylation have been reported, and alternative splicing generates multiple transcript variants.
• The three-dimensional structure has been solved, offering molecular details of ESCRT-I assembly.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 122–221, a region that may confer specificity for VPS28 across human, mouse, and rat.
• For western blot, consider probing whole-cell lysates from human, mouse, or rat cell lines, given the documented cross-reactivity.
• In IHC-P, validate the antibody in tissues with known VPS28 expression (e.g., those rich in endosomal compartments) and optimize antigen retrieval conditions.
• ELISA can be employed to detect recombinant VPS28 protein; ensure the coating antigen includes the epitope region.
• Always include appropriate controls, as performance may vary with sample type and preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-VPS28 antibody supports research in endosomal trafficking and protein sorting, areas relevant to cancer and neurodegenerative diseases. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.