Anti-VPS28 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UK41

Core Product Specifications and Parameters

Biological Background: VPS28 Function and Localization

• VPS28 is the human homolog of yeast Vps28, a core component of the endosomal sorting complexes required for transport (ESCRT)-I complex, also known as the ESCRT-I complex subunit VPS28.
• As part of ESCRT-I, VPS28 functions as a regulator of vesicular trafficking, specifically mediating the sorting of ubiquitinated membrane proteins into multivesicular bodies (MVBs) for lysosomal degradation.
• Subcellular localization studies indicate that VPS28 is associated with the cell membrane and the late endosome membrane, consistent with its role in endosomal cargo sorting.
• The protein undergoes acetylation and is subject to alternative splicing, contributing to functional diversity; it has been identified in proteomics analyses and serves as a reference proteome component.
• The predicted molecular weight of VPS28 is approximately 25 kDa (UniProt: 25425 Da), matching the observed band in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human VPS28 (aa 1–221), which may provide broad epitope coverage. For Western blot, consider starting with a standard dilution range (e.g., 1:500–1:2000) and validate using lysates from human, mouse, or rat cell lines known to express VPS28.
• For IF/ICC, optimize fixation and permeabilization conditions, and include appropriate controls to confirm specificity in endosomal membrane staining.
• In ELISA, pairing this polyclonal antibody with a matched detection antibody may enhance sensitivity; empirically determine coating antigen concentrations and antibody titers.
• Given the antibody's cross-reactivity with mouse and rat, it is suitable for comparative studies across these species, but always verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of antibodies, antigen pairs, and ancillary reagents—including this VPS28 polyclonal antibody for ESCRT-I and vesicular trafficking studies. Whether you need validated antibody pairs for immunoassay development, bulk monoclonal/polyclonal reagents, or assistance sourcing rare-target raw materials, our integrated supply chain and technical consulting support your transition from concept to clinic. Partner with CamelBio to efficiently bridge discovery and diagnostic manufacturing.