Anti-VPS26A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75436

Core Product Specifications and Parameters

Biological Background: VPS26A Function and Localization

• VPS26A, also known as hVPS26 or HB58, is a core component of the retromer cargo-selective complex (CSC), which prevents missorting of transmembrane cargo to the lysosome and mediates endosome-to-TGN and plasma membrane recycling.
• The retromer CSC is recruited to the endosomal membrane via RAB7A and SNX3, acting as a hub for accessory proteins such as the WASH complex and TBC1D5.
• VPS26A is required for retrograde transport of the lysosomal enzyme receptor IGF2R, a process critical for lysosomal biogenesis. Related references: PMID:15078902 PMID:15078903
• It regulates transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA), linking retromer function to mucosal immunity. Related references: PMID:15247922
• VPS26A mediates endosomal localization of WASHC2A and TBC1D5, coordinating actin dynamics and GTPase signaling. Related references: PMID:22070227 PMID:20923837
• It recognizes and traffics the Alzheimer’s-related receptor SORL1, influencing APP processing. Related references: PMID:22279231
• Beyond retromer, VPS26A participates in retromer-independent lysosomal sorting of F2R and recycling of ADRB2. Related references: PMID:16407403 PMID:21602791
• Subcellularly, it localizes to the cytoplasm, endosome membrane, and early endosomes, and is regulated by phosphorylation (phosphoprotein) and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 256–327, a C-terminal region potentially exposed in the native complex. For Western blot, expect a band near 38 kDa. Consider optimizing blocking and antibody dilutions for endogenous detection.
• In immunofluorescence/ICC, ensure proper fixation and permeabilization to access endosomal compartments. Co-staining with endosomal markers (e.g., EEA1) may confirm localization.
• Since this antibody cross-reacts with mouse and rat, it can be used for interspecies studies, but validate in the relevant sample system due to possible isoform variation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD manufacturers and researchers by providing one-stop access to critical raw materials. This anti-VPS26A antibody supports investigations into endosomal trafficking and retromer biology, and we can source validated antibody pairs, bulk ancillary reagents, and custom monoclonals for assay development. Tell us your target—we’ll bridge the supply gap efficiently.