Anti-VPS25 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BRG1

Core Product Specifications and Parameters

Biological Background: VPS25 Function and Localization

• VPS25 (vacuolar protein-sorting-associated protein 25) is a core subunit of the ESCRT-II complex (endosomal sorting complex required for transport II), also known as DERP9, EAP20, or FAP20.
• The ESCRT-II complex is essential for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs, mediating delivery of transmembrane proteins to the lysosome for degradation.
• VPS25 is involved in recruiting the ESCRT-III complex, a downstream component of the MVB pathway.
• The ESCRT-II complex also plays a role in transcription regulation through its interaction with ELL, and may facilitate the budding of certain RNA viruses.
• Subcellular localization includes the cytoplasm, endosome membrane, and nucleus (nucleoplasm).
• At the mRNA level, VPS25 is broadly expressed, with highest levels in kidney, liver, pancreas, and placenta, and lower levels in heart, skeletal muscle, brain, and lung.
• Keywords associated with VPS25 include protein transport, transcription regulation, and endosome membrane dynamics, reflecting its multifunctional roles in cellular trafficking and gene expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human VPS25 sequence (residues 1–176); due to high sequence conservation, the antibody may recognize endogenous VPS25 in human, mouse, and rat samples.
• For Western blot, expect a band around 21 kDa. Optimize blocking and antibody dilution according to the specific sample type.
• In IF/ICC, fixation and permeabilization conditions should be carefully adjusted to preserve VPS25 localization at endosomal membranes and in the nucleus.
• For ELISA, recombinant VPS25 protein or lysates from transfected cells can serve as positive controls; verify cross-reactivity with mouse or rat VPS25 using species-matched samples.
• As VPS25 forms part of the ESCRT-II complex, co-immunoprecipitation experiments may require mild detergents to maintain complex integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to critical IVD raw materials. For researchers investigating endosomal sorting, transcription regulation, or viral budding, we offer optimized polyclonal antibodies like this anti-VPS25 antibody, along with validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Our sourcing network also expedites procurement of rare-target raw materials to support your diagnostic development from concept to clinic.