Anti-VPS18 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P253

Core Product Specifications and Parameters

Biological Background: VPS18 Function and Localization

• VPS18 (vacuolar protein sorting-associated protein 18 homolog), also known as PEP3, is a 110 kDa protein that serves as a core component of the HOPS and CORVET endosomal tethering complexes.
• It plays a critical role in vesicle-mediated protein trafficking to lysosomes, including endocytosis and autophagy, by mediating the Rab5-to-Rab7 endosome conversion and facilitating SNARE-mediated membrane fusion. Related references: PMID:11382755, PMID:23351085, PMID:24554770
• Essential for the fusion of endosomes and autophagosomes with lysosomes, a key step in autophagic degradation. Related references: PMID:25783203
• Subcellularly, VPS18 localizes to late endosomes, lysosomes, early endosomes, autophagosomes, and clathrin-coated vesicles.
• Ubiquitously expressed across tissues, with highest levels observed in heart and lowest in lung.
• Post-translational modifications include acetylation and phosphorylation; the protein contains zinc-finger and coiled-coil domains and binds zinc ions.
• Involved in dendrite development of Purkinje cells, as suggested by similarity data.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a synthetic peptide of human VPS18; verify reactivity in your specific sample type and species.
• Suitable for WB (expected band ~110 kDa), IF/ICC, and ELISA. For IF/ICC, consider fixation and permeabilization conditions given VPS18’s membrane associations.
• Demonstrates cross-reactivity with human, mouse, and rat; sequence homology should be examined for other species if needed.
• As with all polyclonal antibodies, lot-to-lot consistency may vary; validate each new lot in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies for autophagy and endocytosis research. Our VPS18 polyclonal antibody is one of many tools available to support your diagnostic assay development, alongside customized antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or challenging targets, our sourcing services can bridge the gap, streamlining your path from concept to clinic.