Anti-VPS18 Rabbit Monoclonal Antibody for WB and ELISA - Q9P253

Core Product Specifications and Parameters

Biological Background: VPS18 Function and Localization

• VPS18 (Vacuolar protein sorting-associated protein 18 homolog) is a 110 kDa core subunit of the VPS-C complexes, essential for membrane tethering and fusion in the endocytic and autophagic pathways.
• It acts as a central component of the HOPS and CORVET endosomal tethering complexes, which mediate SNARE-dependent membrane fusion and the Rab5-to-Rab7 conversion during endosome maturation (PubMed:11382755, 23351085, 24554770). Related references: PMID:11382755, PMID:23351085, PMID:24554770
• VPS18 is required for the fusion of endosomes and autophagosomes with lysosomes, a critical step in terminal degradation within both endocytic and autophagic routes (PubMed:25783203). Related references: PMID:25783203
• The protein localizes to late endosomes, lysosomes, early endosomes, autophagosomes, and clathrin-coated vesicles, reflecting its broad role in intracellular trafficking.
• VPS18 contains coiled-coil domains and a zinc-finger motif, indicating functions in protein–protein interactions and metal coordination, and is regulated by post-translational modifications including acetylation and phosphorylation.
• It is ubiquitously expressed, with highest levels in heart, suggesting tissue-variable but widespread involvement in vesicular transport.
• VPS18 also contributes to dendrite development in Purkinje cells (By similarity), implicating it in neuronal membrane trafficking.

Experimental Guidance and Technical Tips

• For Western blot, a specific band near 110 kDa is expected; optimize SDS-PAGE conditions and transfer for high molecular weight proteins.
• The immunogen corresponds to amino acids 1-100 of human VPS18; consider using peptide blocking or knockout controls to confirm specificity.
• In ELISA applications, the immunizing peptide may serve as a positive control; indirect ELISA formats are recommended.
• Validate the antibody in human cell lines with known VPS18 expression; enrichment of endosomal/lysosomal fractions may enhance detection.
• As a rabbit monoclonal antibody, this reagent should offer high affinity and consistent lot performance; optimal dilutions and incubation parameters should be determined empirically.

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