Anti-VPS16 Rabbit Polyclonal Antibody for WB and ELISA - Q9H269

Core Product Specifications and Parameters

Biological Background: VPS16 Function and Localization

• VPS16 (vacuolar protein sorting-associated protein 16 homolog), also known as hVPS16 or DYT30, is a core component of the HOPS and CORVET endosomal tethering complexes. It plays a critical role in vesicle-mediated protein trafficking to lysosomal compartments, including the endocytic and autophagic pathways. Related references: PMID:11382755, PMID:23351085, PMID:24554770
• VPS16 is required for the fusion of endosomes and autophagosomes with lysosomes; this function depends on its association with VPS33A (but not VPS33B) and involves STX17 but not UVRAG. Related references: PMID:25783203, PMID:24554770
• The protein localizes to late endosome/lysosome membranes, early endosomes, clathrin-coated vesicles, and autophagosomes, consistent with its role in tethering and fusion events.
• VPS16 is ubiquitously expressed across human tissues, supporting its fundamental role in membrane trafficking.
• Mutations in VPS16 are associated with dystonia (DYT30). The protein undergoes alternative splicing and nitration, and is identified in proteomics reference datasets.
• The full-length human VPS16 protein (839 amino acids) has a predicted molecular mass of approximately 95 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the C‑terminal region (aa 540–839); the polyclonal antibody may recognize full‑length VPS16 and possibly isoforms containing this segment.
• For western blotting, a predominant band near 95 kDa is expected in human, mouse, and rat lysates. Consider using cell lines with moderate to high VPS16 expression (e.g., HeLa, HEK293) as positive controls.
• In ELISA, the antibody can be applied as a capture or detection reagent; optimize coating concentration and blocking conditions for your specific assay format.
• Cross‑reactivity has been confirmed for human, mouse, and rat. Detection in other species may be possible due to sequence conservation but should be validated experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw‑material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. For VPS16‑related projects, we provide one‑stop access to rare‑target antibodies and bulk‑supply solutions tailored to diagnostic development. Let us streamline your workflow from concept to clinic with reliable sourcing and technical support.