Anti-VPS13A Polyclonal Antibody for WB and ELISA - Q96RL7

Core Product Specifications and Parameters

Biological Background: VPS13A Function and Localization

• VPS13A (chorein) is a large intermembrane lipid transfer protein (~360 kDa) that binds phospholipids and mediates non-vesicular lipid transport between organelles at membrane contact sites, such as ER-mitochondria and ER-lipid droplet interfaces. Related references: PMID:34830155 PMID:30741634
• It is required for the formation or stabilization of ER-mitochondria contact sites and negatively regulates lipid droplet size and motility. Related references: PMID:30741634
• VPS13A is necessary for efficient lysosomal protein degradation, implicating it in endolysosomal trafficking. Related references: PMID:30709847
• The protein localizes to multiple organelle membranes, including the mitochondrial outer membrane, endoplasmic reticulum, endosomes, lysosomes, lipid droplets, Golgi apparatus, and neuronal dense core vesicles.
• Expression is broad, with high levels in brain, heart, skeletal muscle, and kidney; it is also detected in red blood cells. Related references: PMID:15498460 PMID:31086825
• VPS13A is associated with the neurodegenerative disorder chorea-acanthocytosis and features TPR repeats, alternative splicing, and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350–600 of human VPS13A; consider whether this region is conserved across isoforms or species if cross-reactivity beyond human is desired.
• For Western blotting, account for the high molecular weight (360 kDa) by using low-percentage acrylamide gels (e.g., 6%) and extended transfer times, and include suitable molecular weight markers.
• Polyclonal format may offer multi-epitope recognition, potentially improving detection in denaturing or native conditions, but batch-to-batch variability should be assessed.
• In ELISA, validate the antibody’s performance with both recombinant protein and relevant cell lysates to ensure specificity for VPS13A.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting your workflow from research to commercialization. For VPS13A—a key lipid transfer protein in organelle contact sites and neurodegeneration—we supply validated polyclonal antibodies and can assist with bulk reagent procurement, matched antibody pairs, and rare-target custom sourcing to meet your specific assay needs. Let our expertise bridge the gap between target discovery and clinical application.