Anti-VPS11 Monoclonal Antibody for WB, IHC-P, ELISA - Q9H270

Core Product Specifications and Parameters

Biological Background: VPS11 Function and Localization

• VPS11, also designated as vacuolar protein sorting-associated protein 11 homolog and RING finger protein 108, is a key component of the endolysosomal trafficking machinery.
• It acts as a core subunit of the HOPS and CORVET endosomal tethering complexes, mediating Rab5-to-Rab7 conversion and SNARE-mediated membrane fusion events. Related references: PMID:11382755 PMID:23351085 PMID:24554770
• VPS11 is required for the fusion of endosomes and autophagosomes with lysosomes, a critical step in the degradation of endocytic and autophagic cargo. Related references: PMID:25783203
• It participates in cargo transport from early to late endosomes and is essential for the early-to-late endosome transition. Related references: PMID:21148287
• VPS11 is involved in the retrograde transport of Shiga toxin, highlighting its role in intracellular trafficking pathways. Related references: PMID:23593995
• Subcellularly, VPS11 localizes to late endosomes, lysosomes, early endosomes, autophagosomes, and clathrin-coated vesicles, consistent with its trafficking functions.
• Expression of VPS11 is ubiquitous, with the highest levels observed in the heart and low levels in the lung.
• The protein contains a zinc-finger domain and undergoes post-translational modifications such as acetylation, methylation, and phosphorylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human VPS11, which is conserved in mouse, supporting the observed cross-reactivity. Consider verifying specificity using knockout cell lines or blocking peptides if non-specific binding is suspected.
• For Western blot (WB), the expected molecular weight is approximately 108 kDa. Ensure adequate protein loading and use appropriate prestained markers for size confirmation.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary due to the membrane association of VPS11. Validate staining in tissues with known high expression, such as heart, and include appropriate controls.
• For ELISA applications, the antibody can be used as a capture or detection reagent. Pairing with a matched detection antibody may improve sensitivity; performance should be experimentally optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibodies against VPS11 for studying endosomal trafficking, autophagy, and related disorders. As a one-stop sourcing partner, we offer monoclonal antibodies, ancillary reagents, and bulk procurement options to streamline your assay development. Whether you need standard catalog products or assistance with rare-target material sourcing, CamelBio is dedicated to supporting your transition from concept to clinic.