Anti-von Willebrand Factor (VWF) Rabbit Monoclonal Antibody for IHC-P, ELISA - P04275

Core Product Specifications and Parameters

Biological Background: von Willebrand factor Function and Localization

• von Willebrand factor (VWF) is a large multimeric glycoprotein essential for hemostasis, encoded by the VWF gene in humans.
• It mediates platelet adhesion to sub-endothelial collagen at sites of vascular injury by forming a bridge between collagen and the platelet GPIb-IX-V receptor complex.
• VWF serves as a specific chaperone for coagulation factor VIII, stabilizing its heterodimeric structure and protecting it from premature proteolytic degradation in plasma.
• Localized to the extracellular matrix and secreted into plasma; synthesized by endothelial cells and megakaryocytes.
• Plasma VWF is derived primarily from endothelial cells and is critical for primary hemostasis.
• Mutations in VWF cause von Willebrand disease, a common inherited bleeding disorder characterized by quantitative or qualitative defects.
• Post-translational modifications include extensive glycosylation and disulfide bond formation, crucial for multimer assembly and function.

Experimental Guidance and Technical Tips

• For IHC-P, consider antigen retrieval steps due to the large size and extracellular matrix localization of VWF. Validate the antibody on positive control tissues (e.g., human vascular endothelium) and mouse tissues, given cross-reactivity.
• In ELISA applications, this rabbit monoclonal antibody can be used as a capture or detection reagent. Pair it with a different clone or polyclonal antibody to avoid epitope competition, and verify performance with recombinant VWF standards.
• As a rabbit monoclonal, it generally provides high specificity and lot-to-lot consistency. However, optimal working dilutions should be determined experimentally in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this VWF rabbit mAb for hemostasis research and assay development. Our portfolio encompasses optimized antibody pairs, recombinant proteins, and bulk ancillary reagents to support coagulation-related diagnostic kit production. For specialized sourcing requirements or technical consultation, CamelBio delivers reliable end-to-end support from concept to clinical application.