Anti-VNN1 Rabbit Polyclonal Antibody for WB and ELISA - O95497

Core Product Specifications and Parameters

Biological Background: VNN1 Function and Localization

• Pantetheinase (VNN1) is an amidohydrolase that specifically cleaves D-pantetheine to recycle pantothenic acid (vitamin B5) and release cysteamine.
• Also known as Vanin-1 or Tiff66, it is a GPI-anchored glycoprotein located on the cell membrane.
• VNN1 is widely expressed, with higher levels in spleen, kidney, and blood; it is overexpressed in lesional psoriatic skin.
• The protein belongs to the biotinidase family and functions as a pantetheine hydrolase.
• Post-translational modifications include a GPI-anchor that tethers it to the outer leaflet of the plasma membrane.
• According to UniProt keywords, VNN1 is a signal-anchored membrane protein with a 3D structure available, indicating potential for structural studies.
• It may play a role in oxidative stress and inflammation due to its cysteamine release.

Experimental Guidance and Technical Tips

• The immunogen (aa 300-415) lies within the C-terminal region; this region may be exposed extracellularly. Consider using it for detection of native protein in cell surface assays.
• For Western blot, a band around 57 kDa is expected; validate the molecular weight against the predicted size and consider potential glycosylation shifts.
• Cross-reactivity with mouse suggests sequence conservation; this antibody may be suitable for mouse model studies, though validation in the relevant system is recommended.
• For ELISA, optimise coating conditions and detection antibodies; the GPI-anchored nature might require membrane protein extraction protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined pathway to high-quality IVD raw materials. For VNN1-related studies in pantetheine metabolism and inflammatory skin diseases, our portfolio includes validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. We also specialize in rare-target raw-material sourcing to accelerate your concept-to-clinic journey.