Anti-Vitronectin (S-protein, V75) Monoclonal Antibody for WB, ELISA - P04004

Core Product Specifications and Parameters

Biological Background: Vitronectin Function and Localization

• Vitronectin, also known as S-protein, V75, or serum-spreading factor, is a multifunctional secreted glycoprotein that comprises a somatomedin-B domain with protease-inhibiting activity and multiple binding sites for integrins, glycosaminoglycans, and proteoglycans.
• It acts as a cell adhesion molecule by binding to integrin receptors, promoting cell-to-substrate adhesion and spreading in serum and the extracellular matrix.
• Vitronectin inhibits the terminal cytolytic complement pathway by binding to the C5b-7 complex, preventing membrane attack complex formation and protecting cells from complement-mediated lysis.
• The protein undergoes several post-translational modifications, including phosphorylation, sulfation, and formation of disulfide bonds, which are likely critical for its structural integrity and function.
• Predominantly expressed in plasma and serum, vitronectin is also detected in the retina pigment epithelium at the protein level.
• Its subcellular localization is primarily extracellular, with reported presence in parasitophorous vacuoles during certain infections.

Experimental Guidance and Technical Tips

• For WB, use a reducing gel system and validate detection in serum or plasma samples, as vitronectin is a secreted protein abundant in these fluids. Expect a band near the predicted 54 kDa molecular weight.
• In ELISA, the antibody may be used as capture or detection. Consider pairing with another clone targeting a non-overlapping epitope for enhanced specificity. Determine optimal dilutions empirically.
• Due to vitronectin's role in complement inhibition and cell adhesion, this antibody is a useful tool for functional studies in immunology and extracellular matrix research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high-quality IVD raw materials like this anti-vitronectin monoclonal antibody, backed by comprehensive technical support. Whether you need validated antibody pairs, bulk quantities of monoclonal or polyclonal antibodies, or specialized ancillary reagents for complement and cell adhesion studies, our sourcing solutions cover the entire product lifecycle. Let us streamline your procurement, from concept to clinic, with a focus on rare target raw-material sourcing and customized supply chains.