Anti-Vitronectin Polyclonal Antibody for WB, IHC-P, ELISA - P04004

Core Product Specifications and Parameters

Biological Background: Vitronectin Function and Localization

• Vitronectin is a multifunctional glycoprotein that promotes cell adhesion and spreading by interacting with integrins, glycosaminoglycans, and proteoglycans.
• It acts as an inhibitor of the terminal cytolytic complement pathway, preventing membrane damage.
• The N-terminal somatomedin-B domain exhibits growth hormone-dependent protease-inhibiting activity.
• Vitronectin is secreted into the extracellular space and has been detected within parasitophorous vacuoles under certain conditions.
• It is abundantly expressed in plasma and serum (PubMed:2448300; PubMed:29567995), and also found in the retina pigment epithelium (PubMed:25136834). Related references: PMID:2448300 PMID:29567995 PMID:25136834
• Post-translational modifications such as glycosylation, phosphorylation, sulfation, and disulfide bond formation are critical for its structural integrity and functional interactions.

Experimental Guidance and Technical Tips

• The immunogen maps to the C-terminal region (aa 364–478); this design may detect both full-length vitronectin and certain cleavage fragments.
• For western blot, the predicted molecular weight is approximately 54 kDa; note that glycosylation and other modifications can cause shifts in apparent MW.
• Cross-reactivity with mouse and rat is confirmed; validation in the respective sample systems is recommended for multi-species studies.
• For immunohistochemistry on paraffin-embedded tissues (IHC-P), consider optimizing antigen retrieval and antibody dilution based on your protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material sourcing for diagnostic developers and research laboratories. For projects targeting vitronectin in cell adhesion, complement inhibition, or protease regulation, we offer validated antibody pairs, optimized monoclonal and polyclonal preparations, bulk ancillary reagents, and rare-target sourcing support. Let CamelBio connect your concept to the clinic.