Anti-Vitamin D Binding Protein Rabbit Polyclonal Antibody for WB, ELISA - P02774

Core Product Specifications and Parameters

Biological Background: Vitamin D-binding Protein Function and Localization

• Vitamin D-binding protein (DBP), also referred to as Gc-globulin or group-specific component (Gc), is a multifunctional plasma protein encoded by the GC gene, existing in several isoforms due to alternative splicing.
• DBP serves as the primary carrier of vitamin D and its metabolites, playing an essential role in their transport, storage, and bioavailability throughout the body.
• Upon tissue injury, DBP binds and clears extracellular G-actin released from damaged cells, preventing the formation of detrimental actin filaments and maintaining microvascular function.
• In the immune system, DBP enhances the chemotactic activity of complement-derived C5a for neutrophils, and can be post-translationally modified to Gc protein-derived macrophage activating factor (Gc-MAF), a potent macrophage activator.
• Predominantly synthesized in the liver and secreted into the bloodstream, DBP is detected in various physiological fluids including plasma, ascites, cerebrospinal fluid, and urine.
• The mature protein has a molecular weight of approximately 53 kDa (precursor ~52.9 kDa) and features multiple structural domains characteristic of the albumin gene family, along with glycosylation and disulfide bonds.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment spanning amino acids 324-493 of human DBP; it may recognize epitopes within this C-terminal region.
• For Western blot (WB), consider using protein lysates from human liver tissue or plasma/serum samples as a positive control; the expected band is ~53 kDa. Cross-reactivity with mouse DBP allows parallel testing in murine models, but validation in the relevant species and tissue is advised.
• In ELISA applications, the antibody can be used as a capture or detection reagent; optimal working dilutions and pairing antibodies must be determined experimentally due to polyclonal batch variability.
• Due to its polyclonal nature, lot-specific validation for your specific assay conditions (e.g., sample matrix, detection method) is strongly recommended to ensure consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, bulk reagents, and customized sourcing for specialized targets like vitamin D-binding protein. Our integrated supply chain and technical consultation support the development of immunoassays for vitamin D metabolism, immune function, and inflammatory diseases. Partner with CamelBio to streamline your raw-material procurement from early-stage research to regulatory submission.