Anti-VISTA/B7-H5/GI24 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H7M9

Core Product Specifications and Parameters

Biological Background: VISTA/B7-H5/GI24 Function and Localization

• VISTA (V-set immunoregulatory receptor), also designated B7-H5, GI24, PD-1H, or SISP1, is a transmembrane protein encoded by the VSIR (C10orf54) gene. It belongs to the immunoglobulin superfamily.
• Acts as an inhibitory immune checkpoint receptor that suppresses T-cell activation (PubMed:24691993). Related references: PMID:24691993
• Has been implicated in the regulation of MMP2 activity via MMP14 (PubMed:20666777), and may modulate BMP4 signaling in embryonic stem cells (By similarity). Related references: PMID:20666777
• Predominantly localizes at the plasma membrane and shows high expression in placenta, spleen, lung, and peripheral blood leukocytes; moderate levels in lymph node, bone marrow, and uterus.
• Abundantly present on myeloid lineage cells (CD11b⁺ monocytes, CD66b⁺ neutrophils) and at lower levels on CD4⁺ and CD8⁺ T cells and a subset of NK cells; protein expression is absent on B cells.
• Contains V-type immunoglobulin domain, a signal peptide, a transmembrane helix, and multiple post-translational modification sites including N‑glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning amino acids 33–194 of human VISTA, corresponding to the extracellular domain. It is expected to recognize the mature form of the protein.
• For Western blot, consider using whole‑cell lysates from human spleen, lung, or activated immune cells where VISTA expression is reported; a band around 33 kDa is anticipated. Validate the antibody in your specific sample system and optimize blocking/detection conditions.
• In IHC‑P, you may need to perform antigen retrieval; include appropriate positive controls (e.g., human placenta or spleen sections) and a negative reagent control to confirm specificity.
• For ELISA, this antibody may be used as a capture or detection reagent after empirical pairing and validation. Cross‑reactivity with rat has been indicated, but confirm reactivity in your assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with one‑stop access to a broad portfolio of IVD raw materials, technical services, and consultative support—from early‑stage development to clinical application. This anti‑VISTA rabbit polyclonal antibody is part of our extensive immune checkpoint inhibitor research reagents, sourced to meet rigorous quality standards. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions for rare targets, helping you accelerate your in‑vitro diagnostic workflows.