Anti-VIRMA/KIAA1429 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, ELISA - Q69YN4

Core Product Specifications and Parameters

Biological Background: VIRMA/KIAA1429 Function and Localization

• VIRMA (virilizer homolog, also known as KIAA1429, MSTP054, and fSAP121) is a large 202 kDa protein encoded by the VIRMA gene in humans.
• It serves as an essential component of the WMM complex, which mediates N6-methyladenosine (m6A) methylation of RNAs, thereby modulating mRNA splicing and processing efficiency. Related references: PMID:24981863 PMID:29507755
• VIRMA acts as a key regulator of m6A methylation by recruiting the catalytic core components METTL3 and METTL14, guiding site-specific methylation primarily in the 3'-UTR near stop codons. Related references: PMID:29507755
• This m6A methylation pattern correlates with alternative polyadenylation (APA), making VIRMA critical for mRNA polyadenylation regulation. Related references: PMID:29507755
• Subcellular localization includes the nucleus speckles, nucleoplasm, and cytoplasm, reflecting its dynamic roles in RNA processing.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and methylation, and is subject to alternative splicing, generating distinct isoforms.
• VIRMA is classified under developmental proteins and has been identified through direct protein sequencing and proteomics analyses.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 221–320 of NP_056311.2) targets an internal region of human VIRMA, ensuring specificity; cross-reactivity with mouse is reported.
• For Western blot detection of VIRMA at ~202 kDa, use high-molecular-weight protein markers and consider extended transfer times; validate in lysates from relevant cell lines overexpressing or endogenously expressing VIRMA.
• In IHC-P, antigen retrieval may be necessary; optimize staining conditions using positive control tissues and validate specificity with knockout or knockdown controls.
• For ELISA, titrate antibody concentrations against the immunogen peptide or recombinant protein to establish optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories a consolidated source for IVD raw materials, including high-quality monoclonal antibodies for epitranscriptomic targets such as VIRMA/KIAA1429. Our inventory spans validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your assay development workflows. Leverage our expertise for rare target sourcing and technical consulting to accelerate your path from concept to clinic.