Anti-VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC-P, ELISA - Q69YN4

Core Product Specifications and Parameters

Biological Background: VIRMA Function and Localization

• VIRMA (Protein virilizer homolog), also known as KIAA1429, MSTP054, or fSAP121, is an associated component of the WMM complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that influences mRNA splicing and processing efficiency. Related references: PMID:24981863 PMID:29507755
• It functions as a key regulator by recruiting the catalytic core methyltransferases METTL3 and METTL14, thereby guiding site-specific m6A methylation predominantly in the 3'-UTR near stop codons. Related references: PMID:29507755
• VIRMA-dependent m6A methylation is required for proper mRNA polyadenylation and correlates with alternative polyadenylation (APA), highlighting its role in 3'-end processing. Related references: PMID:29507755
• Subcellularly, VIRMA localizes to nuclear speckles, the nucleoplasm, and the cytoplasm.
• The protein undergoes post-translational modifications including phosphorylation and acetylation, and is subject to alternative splicing.
• VIRMA is classified as a developmental protein and its sequence has been verified by direct protein sequencing.
• The full-length protein has a predicted molecular weight of approximately 202 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering amino acids 1400–1700 of human VIRMA, which may confer specificity for the target in various assays.
• For western blot (WB), a band around 202 kDa is expected; consider using a low-percentage polyacrylamide gel and prolonged transfer times to ensure efficient blotting of this high-molecular-weight protein.
• IHC-P applications may require optimized antigen retrieval; validation in the intended tissue type and fixation conditions is recommended.
• Cross-reactivity with mouse and rat orthologs makes this antibody suitable for studies in common model organisms, but verify expression levels in the relevant sample system.
• The ELISA application suggests potential utility in quantitative assays; titration should be performed to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive sourcing platform offering IVD manufacturers and research laboratories streamlined access to validated raw materials, technical services, and regulatory consulting. This anti-VIRMA polyclonal antibody exemplifies our commitment to supporting research in epitranscriptomics and RNA modification biology. We provide one-stop solutions including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your diagnostic and research workflows.