Anti-VIPAS39 Rabbit Polyclonal Antibody for WB, ELISA - Q9H9C1

Core Product Specifications and Parameters

Biological Background: VIPAS39 Function and Localization

• VIPAS39 (SPE39, VIPAR, VPS16B) is a spermatogenesis-defective protein 39 homolog encoded by the human gene VIPAS39 (formerly C14orf133). Multiple isoforms exist through alternative splicing.
• In epithelial cells, VIPAS39 forms a complex with VPS33B, playing a critical role in apical RAB11A-dependent recycling endosome trafficking and maintaining apical-basolateral polarity. Related references: PMID:20190753
• VIPAS39 is also implicated in lysosomal trafficking, potentially via interaction with the core HOPS complex in a discrete endosomal population, independent of VPS33B. Related references: PMID:19109425
• By similarity, VIPAS39 has a proposed function in vesicular trafficking during spermatogenesis.
• It may be involved in the transcriptional regulation of E-cadherin (by similarity).
• Subcellular localization includes cytoplasm, cytoplasmic vesicles, early endosomes, recycling endosomes, and late endosomes, consistent with its trafficking roles.
• VIPAS39 is a phosphoprotein and has been linked to protein transport, differentiation, spermatogenesis, and transcription regulation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1–220 of human VIPAS39. This N-terminal region may allow detection of full-length VIPAS39 and possibly some isoforms.
• For Western blotting (WB), the predicted molecular weight is approximately 57 kDa. Validate antibody specificity in appropriate cell or tissue lysates from human or mouse origin.
• ELISA can be used for antibody titer determination or screening; optimal coating conditions and detection systems should be empirically determined.
• Consider including appropriate controls (e.g., knockout or knockdown samples) to confirm specificity in WB.
• Due to its role in endosomal trafficking, experimental conditions that affect endosomal compartments may alter VIPAS39 localization or abundance; plan fixation and permeabilization accordingly if used in immunocytochemistry (not validated by supplier).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical services. This anti-VIPAS39 antibody is part of our extensive catalog for endosomal trafficking and polarity studies, and we can further assist with customized antibody development, bulk supply, and ancillary reagents to accelerate your project from concept to clinic.