Anti-VIP Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P01282

Core Product Specifications and Parameters

Biological Background: VIP Function and Localization

• VIP is a 170-amino acid neuropeptide precursor that generates multiple bioactive peptides, including VIP and PHM-27, through proteolytic processing.
• It activates the VPAC1 and VPAC2 receptors, which are class B1 G protein-coupled receptors, to mediate diverse signaling cascades. Related references: PMID:1318039, PMID:36385145, PMID:8933357
• Widely distributed in the CNS and peripheral nervous system, VIP exerts neuroprotective and immunomodulatory functions. Related references: PMID:3456568
• In the cardiovascular system, VIP causes potent vasodilation and lowers arterial blood pressure. Related references: PMID:15013843, PMID:3654650
• It also relaxes smooth muscle in the respiratory and gastrointestinal tracts, stimulates glycogenolysis, and enhances cardiac contractility. Related references: PMID:15013843
• The related peptide PHM-27 acts as a full agonist at the calcitonin receptor CALCR, mimicking calcitonin effects. Related references: PMID:15013843
• VIP is a secreted protein with key post-translational modifications including amidation and phosphorylation, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• For WB analysis, a band around 19 kDa corresponds to the full-length precursor; smaller processed peptides may require optimized conditions for detection.
• In IHC-P and IF/ICC, consider that VIP is a secreted peptide; staining may appear granular in neuronal or endocrine cells; include appropriate controls.
• Validate the antibody's performance in your specific tissue or cell type, as expression levels vary across CNS and peripheral tissues.
• For ELISA, the antibody can be used as capture or detection depending on the pair design; test against recombinant VIP standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality IVD raw materials for neuroscience and cardiovascular research, including validated monoclonal antibodies like this anti-VIP clone. We supply optimized antibody pairs, bulk ancillary reagents, and sourcing for rare targets to accelerate your assay development. Our integrated supply chain ensures consistent quality and reliable delivery for your diagnostic projects.