Anti-Vinculin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P18206

Core Product Specifications and Parameters

Biological Background: Vinculin Function and Localization

• Vinculin is an actin filament (F-actin)-binding protein crucial for cell-matrix adhesion and cell-cell adhesion, regulating E-cadherin surface expression and potentiating mechanosensing.
• A muscle-specific splice variant, metavinculin, is generated from the VCL gene and plays specialized roles in cardiac and skeletal muscle.
• Vinculin localizes to focal adhesions, adherens junctions, podosomes, and the perinuclear region, linking the actin cytoskeleton to the plasma membrane via sarcolemma association.
• The protein undergoes post-translational modifications, including palmitoylation and phosphorylation, which are thought to regulate its adhesive functions and interaction with binding partners.
• By bridging adhesion sites to the actin network, vinculin influences cell morphology and locomotion, processes essential for wound healing and development.
• Mutations in VCL are associated with inherited cardiomyopathies, highlighting its critical role in maintaining cardiac muscle integrity.

Experimental Guidance and Technical Tips

• For Western blot, the expected molecular weight is approximately 124 kDa for vinculin; a higher band around 150 kDa corresponding to metavinculin may be detected in muscle samples.
• When performing immunofluorescence or immunocytochemistry, permeabilize cells to visualize vinculin at focal adhesions; paraformaldehyde fixation is generally suitable.
• Validate antibody specificity in your sample system using knockout or knockdown controls where possible.
• For ELISA applications, consider optimizing the coating buffer and detection conditions to suit the specific immunoassay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials, supporting every stage from concept to clinic. This anti-vinculin antibody serves as a robust tool for studies in cell adhesion, cytoskeletal organization, and cardiac disease. CamelBio's comprehensive portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing to accelerate your assay development and biomarker research.