Anti-Vinculin Monoclonal Antibody for WB, IHC-P, ELISA - P18206

Core Product Specifications and Parameters

Biological Background: Vinculin Function and Localization

• Vinculin is a highly conserved actin filament (F-actin)-binding protein, with a muscle-specific splice variant known as metavinculin (MV).
• It plays a central role in cell-matrix and cell-cell adhesions by linking integrin adhesion receptors or cadherin complexes to the cortical actin cytoskeleton.
• Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex at adherens junctions.
• The protein localizes to multiple subcellular sites, including focal adhesions, adherens junctions, the cortical cytoskeleton, podosomes, and the perinuclear region.
• In muscle cells, metavinculin is specifically expressed and associates with the sarcolemma.
• Post-translational modifications include acetylation, phosphorylation, and palmitoylation, which modulate its function and localization.
• Mutations in vinculin are associated with cardiomyopathies, and the protein participates in cell morphology and locomotion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1000-1100); for optimal detection, ensure that epitope masking is avoided during sample preparation and fixation.
• In Western blotting (WB), vinculin migrates at approximately 124 kDa; consider using a mid-percentage polyacrylamide gel for clear resolution.
• For immunohistochemistry (IHC-P), validate antigen retrieval and permeabilization conditions to achieve consistent staining patterns.
• Given cross-reactivity with mouse and rat samples, the antibody can be employed for comparative studies across these species; confirm reactivity in the relevant model system.
• When using ELISA, evaluate coating and blocking conditions to minimize background and maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers focused on vinculin and cell adhesion pathways, CamelBio provides reliable access to rare-target raw materials and technical resources. Our supply chain solutions are designed to support efficient procurement and smooth transition from assay development to production.