Anti-Vinculin [KD Validated] Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P18206

Core Product Specifications and Parameters

Biological Background: Vinculin Function and Localization

• Vinculin is an actin filament (F-actin)-binding protein encoded by the VCL gene; a muscle-specific isoform, metavinculin (MV), is generated by alternative splicing.
• It is essential for both cell-matrix adhesion and cell-cell adhesion, regulating cell-surface E-cadherin expression and enhancing mechanosensing by the E-cadherin complex.
• Vinculin localizes to multiple subcellular structures, including adherens junctions, focal adhesions, the cytoskeleton, sarcolemma, podosomes, and the perinuclear cytoplasm.
• The protein is subject to acetylation, phosphorylation, and palmitoylation, which likely modulate its activity and membrane association.
• Disease variants in VCL are associated with cardiomyopathies; metavinculin is specifically implicated in muscle disorders such as dilated cardiomyopathy (CMD1W) and hypertrophic cardiomyopathy (CMH15).
• Vinculin plays roles in cell morphology and locomotion, contributing to dynamic remodeling of the actin cytoskeleton during migration.
• Structural studies indicate vinculin contains repeating domains that enable interactions with multiple binding partners, serving as a molecular scaffold at adhesion sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–258 of human vinculin (N-terminal region); therefore, the antibody may detect full-length vinculin (~124 kDa) and the metavinculin isoform (~150 kDa) in muscle samples.
• For Western blotting, we recommend running appropriate size markers and loading controls; consider using lysates from human, mouse, or rat cell lines that express vinculin. KD validation confirms specificity, but end-users should verify in their own experimental system.
• In immunofluorescence and immunohistochemistry, vinculin typically appears as distinct puncta at focal adhesions and continuous bands at cell-cell junctions. Include negative controls (e.g., vinculin-knockdown cells) and co-staining with adhesion markers to confirm localization.
• For ELISA applications, the antibody can be used in both direct and sandwich formats; optimize coating concentrations and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing of IVD raw materials, offering research-validated antibodies like this anti-Vinculin monoclonal antibody to accelerate your cell adhesion and cytoskeletal studies. Our portfolio includes validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets—supporting you from concept to clinic. Partner with us for reliable, one-stop access to the critical materials your diagnostic and research programs demand.