Anti-Vimentin Rabbit Monoclonal Antibody [KD Validated] for WB, IP, IF/ICC, IHC-P, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a class-III intermediate filament protein encoded by the VIM gene, essential for maintaining cytoskeletal integrity in mesenchymal and other non-epithelial cells.
• It participates in cell directional movement, orientation, and cell sheet organization, and contributes to Golgi complex polarization at the cell migration front.
• Vimentin protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner.
• It is involved with LARP6 in the stabilization of type I collagen mRNAs (COL1A1 and COL1A2), influencing extracellular matrix production.
• Subcellularly, vimentin is found in the cytoplasm, cytoskeleton, nucleus matrix, cell membrane, and axonal projections.
• Highly expressed in fibroblasts, with some expression in T- and B-lymphocytes; often upregulated in hormone-independent mammary carcinoma cell lines.
• The protein undergoes multiple post-translational modifications including acetylation, phosphorylation, S-nitrosylation, and ubiquitination, and is linked to diseases such as cataract.
• Vimentin also plays roles in host-virus interactions and may promote axon outgrowth and motor fiber repair via DSP-mediated recruitment.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 367–466 of human vimentin (P08670); this C-terminal region is conserved, supporting the reported cross-reactivity with mouse and rat.
• For Western blot, the antibody is KD-validated, indicating it can specifically detect endogenous vimentin; consider including a knockdown control in your experiments.
• In immunoprecipitation, the antibody may pull down vimentin along with interacting partners like LARP6 or cytoskeletal components; optimize conditions accordingly.
• For immunofluorescence and immunohistochemistry, validate fixation and permeabilization methods to preserve intermediate filament architecture.
• ELISA applicability suggests the antibody can be used for quantitative detection; optimize coating and detection conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to essential IVD raw materials, including validated monoclonal and polyclonal antibodies like this vimentin antibody, tailored for cytoskeletal and cancer research applications. As a one-stop sourcing partner, CamelBio offers comprehensive solutions from concept to clinic, featuring optimized antibody pairs, bulk ancillary reagents, and sourcing of rare target raw materials to accelerate your diagnostic development.