Anti-Vimentin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a class-III intermediate filament protein encoded by the VIM gene, widely expressed in mesenchymal cells and certain other non-epithelial cell types.
• It forms filamentous networks that attach to the nucleus, endoplasmic reticulum, and mitochondria, contributing to cellular structural integrity and organelle positioning.
• Vimentin plays a critical role in cell directional movement, orientation, and organization of cell sheets, and is involved in Golgi complex polarization at the leading edge of migrating cells.
• It protects the polarity protein SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-dependent manner.
• Vimentin may promote axon outgrowth and motor fiber repair by recruiting DSP to outgrowth tips.
• Together with LARP6, vimentin stabilizes type I collagen mRNAs (CO1A1 and CO1A2), linking the cytoskeleton to extracellular matrix production.
• It localizes to the cytoplasm, cytoskeleton, nucleus matrix, cell membrane, and axon projections, reflecting its dynamic roles in cellular processes.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and ubiquitin-like conjugation, which regulate its assembly and interactions. It is also implicated in cataract formation and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–200 of human Vimentin, targeting the N-terminal region; confirm antigen specificity in your assay.
• For Western blot, a band around 54 kDa is expected; consider using whole cell lysates from fibroblasts or mesenchymal cell lines as positive controls.
• In IHC-P, optimize antigen retrieval and antibody dilution; cross-reactivity with mouse and rat makes it suitable for comparative tissue studies.
• For IF/ICC, fixation and permeabilization protocols may affect filament visualization; test conditions to preserve intermediate filament structure.
• The antibody is polyclonal, so lot-to-lot consistency should be verified when establishing critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including validated anti-Vimentin polyclonal antibodies for diverse assay platforms. With expertise in antibody development, ancillary reagent supply, and sourcing of rare targets, we support your workflow from early R&D to clinical application. Contact us to explore bulk and customized sourcing options for your specific project needs.