Anti-Vimentin (phospho S83) Rabbit Polyclonal Antibody for WB and ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a class-III intermediate filament protein, predominantly expressed in mesenchymal cells and other non-epithelial cell types.
• Functions as a key cytoskeletal component, providing mechanical support, maintaining cell shape, and facilitating cell migration, directional movement, and Golgi complex polarization at the migration front.
• Partners with LARP6 to stabilize type I collagen mRNAs (CO1A1 and CO1A2), highlighting a role in extracellular matrix regulation.
• Subcellular localization spans the cytoplasm, cytoskeleton, nuclear matrix, cell membrane, and cell projections such as axons.
• Phosphorylation at serine 83 (S83) is a critical regulatory post-translational modification that modulates vimentin filament dynamics and cellular functions.
• The protein undergoes extensive covalent modifications, including acetylation, glycation, S-nitrosylation, and ubiquitination, reflecting tight control in health and disease.
• Highly expressed in fibroblasts, with lower levels in T- and B-lymphocytes, and minimal expression in Burkitt's lymphoma lines; also found in many hormone-independent mammary carcinoma cell lines.

Experimental Guidance and Technical Tips

• For Western blotting, enrich phosphorylated vimentin by including phosphatase inhibitors in the lysis buffer to preserve the S83 modification.
• Confirm antibody phospho-specificity in ELISA by comparing binding to phosphorylated versus non-phosphorylated peptides.
• The broad species reactivity (human, mouse, rat) makes this antibody suitable for translational studies; validate in the specific sample system prior to pivotal experiments.
• Pair with total vimentin antibodies to normalize phosphorylation signals and assess stoichiometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with a comprehensive IVD raw-material portfolio, from validated antibody pairs and optimized recombinant proteins to bulk ancillary reagents. This phospho-vimentin (S83) rabbit polyclonal antibody exemplifies our commitment to supplying precise, application-ready tools for cytoskeleton and cell migration studies. Whether you need routine antibodies or rare-target sourcing, our end-to-end support accelerates your path from concept to clinic.