Anti-Villin1 Monoclonal Antibody for WB, IHC-P, ELISA - P09327

Core Product Specifications and Parameters

Biological Background: Villin1 Function and Localization

• Villin-1 (VIL, D2S1471) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates microvillar actin reorganization.
• It exhibits multiple actin-regulatory activities, including nucleation, bundling, capping, and severing, regulated by Ca2+, PIP2, and LPA binding.
• Binding to LPA enhances SRC-mediated phosphorylation and inhibits actin-modifying activities, while PIP2 inhibits capping/severing but promotes bundling.
• Villin-1 regulates epithelial cell morphology, migration, invasion, and apoptosis; it protects against DSS-induced apoptosis by preserving mitochondrial integrity.
• It enhances HGF-induced epithelial cell motility, chemotaxis, and wound repair.
• During S. flexneri infection, its actin-severing activity facilitates bacterial intracellular movement and dissemination.
• Subcellularly localized to cytoplasm, cytoskeleton, and actin-rich projections: microvilli, lamellipodia, ruffles, filopodia tips.
• Tissue specificity: major component of microvilli in intestinal and kidney proximal tubule epithelia; also expressed in hepatocyte canalicular microvilli.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human Villin1 protein; the resulting monoclonal antibody may recognize linear and conformational epitopes, supporting both denaturing (WB) and native (IHC, ELISA) applications.
• For Western blot, a reducing SDS-PAGE is recommended; expect a principal band at ~93 kDa. Validate specificity using positive (e.g., intestinal or renal epithelial lysates) and negative control cell lines.
• For immunohistochemistry on paraffin sections, antigen retrieval methods (e.g., citrate buffer, pH 6.0) may be required; validate on tissues with high Villin1 expression such as small intestine or kidney.
• The antibody cross-reacts with mouse and rat Villin1, enabling its use in rodent models; confirm species-specific reactivity by comparing with known expression patterns.
• For ELISA, consider using the antibody as capture or detection reagent with recombinant Villin1 or cell/tissue extracts; pair with a non-overlapping anti-Villin1 antibody if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing researchers and manufacturers with validated antibodies, antigens, and ancillary reagents. This anti-Villin1 monoclonal antibody supports studies in epithelial cell biology, gastrointestinal disease, and cytoskeletal dynamics. Beyond this antibody, CamelBio offers bulk procurement, customized antibody development, and technical consultation to streamline your diagnostic pipeline from concept to clinic.