Anti-Villin-like Protein (VILL) Polyclonal Antibody for WB, IF/ICC, ELISA - O15195

Core Product Specifications and Parameters

Biological Background: Villin-like Protein Function and Localization

• Villin-like protein (VILL) is an actin-binding protein belonging to the villin/gelsolin family, encoded by the VILL gene.
• It contains calcium-sensitive actin-capping and actin-severing domains, as indicated by keywords from UniProt.
• VILL may function as a tumor suppressor, though experimental evidence is limited and requires further validation.
• The protein is ubiquitously expressed across 16 human tissues examined, suggesting a broad regulatory role.
• Alternative splicing generates multiple isoforms, potentially influencing its functional diversity.
• VILL has been identified in proteomics studies as part of the reference human proteome.
• Its molecular weight is approximately 96 kDa (UniProt: 95907 Da), consistent with the polyclonal antibody detection range.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 637-856) of human VILL, which shares high homology with mouse and rat orthologs, supporting the observed cross-reactivity.
• For Western blot, expect a predominant band near 96 kDa in whole cell or tissue lysates; validate with known positive controls.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions for the specific cell line, as actin-binding proteins may require careful sample preparation.
• As a polyclonal antibody, each lot may exhibit slight variability; consider routine validation with the relevant sample system before large-scale experiments.
• The antibody is also suitable for ELISA; consider using recombinant VILL protein as a standard for quantitative assays.

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