Anti-Villin-1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P09327

Core Product Specifications and Parameters

Biological Background: Villin-1 Function and Localization

• Villin-1 (VIL1, also known as VIL or D2S1471) is an epithelial cell‑specific actin‑modifying protein encoded by the VIL1 gene in humans.
• It regulates actin dynamics by nucleating actin polymerization, bundling actin filaments, capping the barbed ends, and severing filaments; these activities are modulated by Ca²⁺, phosphatidylinositol 4,5‑bisphosphate (PIP2), and lysophosphatidic acid (LPA).
• Villin-1 is a major structural component of microvilli in intestinal and kidney proximal tubule epithelial cells and is also present in the canalicular microvilli of hepatocytes.
• Subcellularly, it localizes to the cytoplasm, cytoskeleton, and the tips and shafts of several actin‑rich cell projections including microvilli, filopodia, lamellipodia, and membrane ruffles.
• It plays critical roles in maintaining epithelial cell morphology, promoting cell migration and invasion, and protecting against apoptosis in the gastrointestinal epithelium, likely by preserving mitochondrial integrity.
• Villin-1 enhances hepatocyte growth factor (HGF)‑induced epithelial cell motility, chemotaxis, and wound repair.
• Upon infection with Shigella flexneri, its actin‑severing activity is exploited to enhance bacterial motility and dissemination within the host cell.
• The protein undergoes alternative splicing and phosphorylation; LPA binding triggers SRC‑mediated phosphorylation, which inhibits all actin‑modifying functions.

Experimental Guidance and Technical Tips

• The immunogen consists of the full‑length human Villin‑1 protein (amino acids 1‑827), allowing the antibody to potentially recognize both unphosphorylated and phosphorylated forms; validate detection of relevant phospho‑states in your model system.
• For Western blot (WB), expect a single band around 93 kDa; the antibody is reactive with human, mouse, and rat samples, making it suitable for cross‑species studies.
• For immunofluorescence on fixed/permeabilized cells (IF‑P), Villin‑1 will typically label microvilli and other actin‑rich projections; optimize fixation and permeabilization conditions for your cell type.
• For immunohistochemistry on paraffin‑embedded sections (IHC‑P), antigen retrieval may be required; test standard citrate or EDTA‑based protocols.
• For ELISA, consider using this antibody as a capture or detection reagent; pair with a compatible detection antibody for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti-Villin‑1 monoclonal antibody for gastrointestinal and epithelial cell biology research. Beyond this antibody, we can supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare‑target raw materials to accelerate your assay development and scale‑up needs.