Anti-βIII-Tubulin (TUBB3) Monoclonal Antibody for WB, IP, IF, IHC, ELISA - Q13509

Core Product Specifications and Parameters

Biological Background: βIII-Tubulin (TUBB3) Function and Localization

• βIII-Tubulin (TUBB3) is a neuronal-specific class III beta-tubulin isotype that assembles into microtubules, the essential cytoskeletal filaments composed of alpha- and beta-tubulin heterodimers.
  Related references: PMID:34996871, PMID:38305685, PMID:38609661
• TUBB3 plays a critical role in axon guidance and maintenance, directing proper growth cone dynamics in developing neurons.
  Related references: PMID:20074521
• Binding of the guidance cue netrin-1 (NTN1) to its receptor UNC5C triggers dissociation of UNC5C from polymerized TUBB3 in microtubules, enhancing microtubule dynamics and mediating axonal repulsion.
  Related references: PMID:28483977
• During dorsal root ganglion (DRG) development, TUBB3 contributes to the projection of sensory axons toward the spinal cord, a process regulated by netrin-1/UNC5C signaling.
  Related references: PMID:28483977
• Subcellularly, TUBB3 localizes to the cytoplasm and enriches in dynamic cell protrusions such as growth cones, lamellipodia, and filopodia.
• TUBB3 expression is normally restricted to the central and peripheral nervous system, but it is significantly upregulated in most cancerous tissues, making it a cancer biomarker.
• The protein undergoes various post-translational modifications, including phosphorylation, and is subject to alternative splicing; disease-associated mutations in TUBB3 are linked to intellectual disability and congenital fibrosis of the extraocular muscles (CFEOM).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 351–450, covering a unique C-terminal region of βIII-tubulin; this may provide isotype specificity against other beta-tubulin family members.
• For Western blotting, a single band near 50 kDa is expected; consider using knockout validation controls to confirm band identification.
• The antibody is applicable to immunofluorescence (IF-P) and immunohistochemistry (IHC-P); optimal fixation and permeabilization conditions should be empirically determined for each sample type, as microtubule antigens can be sensitive to over-fixation.
• For immunoprecipitation, the rabbit monoclonal format offers high affinity and low background; pre-clearing of lysates and gentle detergent conditions are recommended.
• Cross-reactivity with mouse allows the antibody to be used in common mouse models of neurobiology and cancer; validate in the relevant tissue or cell line system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting. For neuronal and cancer biomarkers such as βIII-tubulin, our portfolio includes validated monoclonal antibodies, recombinant proteins, and ancillary reagents. Whether you need to scale up a WB or IHC assay or source rare target materials, CamelBio streamlines your supply chain from concept to clinic.