Anti-VGLUT2 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, and ELISA - Q9P2U8

Core Product Specifications and Parameters

Biological Background: VGLUT2 Function and Localization

• VGLUT2 (Vesicular glutamate transporter 2), encoded by the SLC17A6 gene, is a ~64 kDa multi-transmembrane glycoprotein belonging to the solute carrier 17 family.
• It functions as a multifunctional transporter for L-glutamate and multiple ions such as chloride, protons, potassium, sodium, and phosphate (PubMed:11698620, PubMed:33440152). Related references: PMID:11698620, PMID:33440152
• At the synaptic vesicle membrane, VGLUT2 acts as a uniporter that preferentially transports L-glutamate and phosphate into vesicles, driven by the electrical gradient established by the vacuolar H+-ATPase (PubMed:11698620). Related references: PMID:11698620
• The protein also exhibits chloride channel activity, facilitating chloride permeation to aid synaptic vesicle acidification, and functions as a vesicular K+/H+ antiporter to sustain glutamate uptake (By similarity).
• Following exocytosis, VGLUT2 localizes to the plasma membrane where it operates as an electrogenic Na+/phosphate symporter, contributing to synaptic phosphate homeostasis (PubMed:10820226). Related references: PMID:10820226
• Subcellularly, VGLUT2 is primarily found in synaptic vesicle membranes, synaptosomes, and at the plasma membrane of excitatory presynaptic terminals.
• It is predominantly expressed in the adult brain, with high levels in regions such as the cerebral cortex, hippocampus, thalamus, and substantia nigra (PubMed:10820226). Related references: PMID:10820226
• In addition to the central nervous system, VGLUT2 may play roles in endocrine glutamatergic systems in the pineal gland and pancreas (By similarity).

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 483-582 of human VGLUT2, a region predicted to be intracellular. For Western blot, consider using standard reducing conditions and expect a band around 64 kDa. Validate in relevant positive controls such as brain lysates from human, mouse, or rat.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC, IF-F, IF-P), optimization of antigen retrieval and permeabilization may be required. Tissue-specific expression patterns should be confirmed in brain sections.
• In ELISA applications, this antibody may be used as a capture or detection reagent; pairing with other clones or detection systems should be empirically determined.

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