Anti-VGLUT1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9P2U7

Core Product Specifications and Parameters

Biological Background: VGLUT1 Function and Localization

• VGLUT1, also known as SLC17A7 or BNPI, is a vesicular glutamate transporter that loads L-glutamate into synaptic vesicles at excitatory presynaptic terminals.
• It functions as an electrogenic uniporter for L-glutamate and phosphate, driven by the proton electrochemical gradient generated by vacuolar H+-ATPase.
• In addition, VGLUT1 exhibits chloride channel activity, facilitating chloride permeation that affects vesicular acidification.
• It may also operate as a K+/H+ antiporter to sustain glutamate uptake by maintaining the vesicular electrical and chemical gradients.
• At the plasma membrane, following exocytosis, VGLUT1 acts as a Na+/phosphate symporter, contributing to synaptic phosphate homeostasis (PubMed:10820226). Related references: PMID:10820226
• The protein is primarily localized to synaptic vesicle membranes and can transit to the presynaptic plasma membrane upon exocytosis.
• It is predominantly expressed in various brain regions, including the amygdala, cerebellum, cerebral cortex, hippocampus, and others.
• Post-translational modifications include phosphorylation, as supported by the keyword "Phosphoprotein".

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1‑100) of human VGLUT1; consider this epitope location when interpreting results.
• For WB, a band around 62 kDa is expected; validate in brain tissue lysates (e.g., hippocampus, cortex) as a positive control.
• For IF-P, use appropriate permeabilization and blocking steps; confirm localization in neuronal samples.
• ELISA can be used to gauge antibody titer; include a non‑immunized rabbit IgG control.
• Cross‑reactivity with mouse and rat is listed; however, sequence alignment of the immunogen region across these species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering one‑stop access to development‑grade antibodies, reagents, and technical support from concept to clinic. This anti‑VGLUT1 antibody is ideal for neuroscience and synaptic research applications; we can source validated antibody pairs, optimized polyclonal or monoclonal options, bulk ancillary reagents, and assistance with rare‑target procurement to streamline your diagnostic or research project.