Anti-Vgll4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q80V24

Core Product Specifications and Parameters

Biological Background: Vgll4 Function and Localization

• Vgll4, or transcription cofactor vestigial-like protein 4 (also known as VGL-4), is a member of the vestigial-like protein family that acts as a specific coactivator for mammalian TEAD transcription factors (TEFs).
• The protein is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.
• According to UniProt keywords, Vgll4 is a phosphoprotein and has a determined 3D structure, indicating it is subject to post-translational modifications that may regulate its activity.
• It is classified as a reference proteome component and participates in transcription regulation pathways, suggesting a conserved function across species.
• While the provided UniProt record focuses on mouse Vgll4, the antibody shows cross-reactivity with human and rat orthologs, enabling multi-species studies.
• Due to limited functional annotations, further research may be needed to fully elucidate Vgll4's specific target genes and regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers the N-terminal region (aa 1-287) of mouse Vgll4, which may be important for recognizing both full-length and potential isoforms across species.
• For Western blotting, an expected band around 31 kDa should be observed; consider using appropriate controls to confirm specificity in the chosen sample system.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution based on tissue type and fixation method; validation in relevant human, mouse, or rat tissues is recommended.
• For ELISA, pre-test with recombinant Vgll4 protein or lysates from transfected cells to establish assay conditions.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments; consider using a blocking buffer appropriate for the detection matrix to minimize non-specific binding.
• This antibody is suitable for studying Vgll4's role in TEAD-mediated transcription, but its performance in other applications like ChIP or IF should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents.
• For researchers investigating transcription cofactors like Vgll4, CamelBio facilitates access to rare-target antibodies and can assist with custom reagent sourcing to support diverse assay development needs.
• Whether you need this anti-Vgll4 rabbit polyclonal antibody for WB, IHC-P, or ELISA, or require larger quantities for kit production, CamelBio streamlines procurement from concept to clinic.