Anti-VGLL4 Polyclonal Antibody for WB, ELISA - Q14135

Core Product Specifications and Parameters

Biological Background: VGLL4 Function and Localization

• VGLL4 (vestigial-like family member 4) is a transcription cofactor that specifically interacts with TEF transcription factors to modulate gene expression.
• The protein contains conserved domains characteristic of the vestigial-like family, enabling its role as a transcriptional coactivator.
• It localizes predominantly to the nucleus, aligning with its function in transcriptional regulation.
• VGLL4 undergoes multiple post-translational modifications, including acetylation and phosphorylation, which may influence its activity and stability.
• Alternative splicing of the VGLL4 gene produces several isoforms, potentially expanding its functional diversity.
• The protein has been identified in large-scale proteomic studies, highlighting its widespread expression and potential roles in various cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 96-296 of human VGLL4, which includes conserved regions; cross-reactivity with mouse and rat suggests this region is well conserved.
• For Western blot, use standard techniques and validate in cell lines or tissues expressing VGLL4; a band around 31 kDa is expected.
• When using ELISA, consider direct or indirect coating methods and optimize antibody and antigen concentrations for your specific assay format.
• As a polyclonal antibody, it may detect multiple isoforms or degradation products; verify specificity using appropriate controls.
• Given its nuclear localization, nuclear extraction or fractionation may enhance detection in some sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and researchers by providing a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For transcription-related targets like VGLL4, we offer reliable polyclonal antibodies such as this one, with cross-species reactivity, to streamline your assay development. From concept to clinic, CamelBio is your trusted sourcing partner, ensuring timely access to high-quality materials for your research and diagnostic needs.